participant blogs - SSP International https://summerscience.org "The educational experience of a lifetime"...since 1959 Wed, 31 Jul 2024 07:46:49 +0000 en-US hourly 1 https://wordpress.org/?v=6.4.5 NMSU Genomics – Day 25 (last one!): Vageesh’s Blog https://summerscience.org/nmsu-genomics-day-25-last-one-vageeshs-blog/ Wed, 31 Jul 2024 07:46:49 +0000 https://summerscience.org/?p=23292 Friday Today, I began my day walking back to Pinon at 12:30 AM after a long day of bioinformatics.  As […]

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Friday

Today, I began my day walking back to Pinon at 12:30 AM after a long day of bioinformatics. 

As usual, Brett, Kano, and I walked to Foster for the morning lectures. We attended lectures from Ms. Alexa, Ms. Ceilidh, Mr. Shakil, and Mr. Joey. Today’s lectures were quite interesting from the line dancing in Ms. Alexa’s lectures to Ms. Ceilidh’s hip-hop dance tutorial. We still had to finish our posters to be submitted for printing, so I spent my lunch looking over the sequences for any potential mutations. 

After lunch, Dr. Duffy’s lecture horrified me for the hopeless future of antibiotics. The lecture made it seem we are all going to die due to the antibiotic resistant bacteria. However, our group felt especially relieved after a few all-day work sessions once the poster was sent to be printed. The rest of the day was spent relaxing and talking to other genomics participants.

Sunday

6 AM, me and Brettt woke up to my peaceful yet horrifying alarm. I wanted to go on a hike at least once during my stay at NMSU, so I was looking forward to this astounding experience. The first 3 hours were easy as the terrain was mostly smooth, but when we started to climb the mountain, that’s where things got really difficult. We were on all fours, desperately clinging to the few but stable boulders that ensured our safety on the rugged mountain side. The view once we reached the top was spectacular, validating the hard work and persistence of our climb. 

Photos were taken courtesy of Lucas Beltran.

The worst part of the hike was yet to come. While climbing down the mountain, I slid over 20 times, nearly slide tackling Emily off the side of the cliff. The limited water in my bag ran out during the first half of the hike, so the next 2 hrs were a living nightmare, with the excessive dehydration and cramps on my body. At the end of the trip, I looked down at my shoes, worn-out and torn, rethinking my choices for this hike. However, putting aside all the dreadful experiences I faced, I consider this hike to be one of the most rewarding things I’ve done, as I persisted through and accomplished a 10 mile hike without any prior experience.

Photo taken by Dr. Messner halfway up the mountain.

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NMSU Genomics – Day 24: Emily’s Blog https://summerscience.org/nmsu-genomics-day-24-emilys-blog/ Wed, 31 Jul 2024 07:43:30 +0000 https://summerscience.org/?p=23288 I woke up to a peaceful morning. Awakening to the blaring eagle screeching that I call my alarm, I walked […]

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I woke up to a peaceful morning. Awakening to the blaring eagle screeching that I call my alarm, I walked into the echoes of hysterical laughter of my suitemates, Angelina, Ritisha, and Chloe. What were they laughing at? I stared at our toilet, overflowing with soapy water (I hope). Turns out, sleep deprived Angelina found through Reddit that the solution to our clogged toilet was a surfactant that we considered shampoo. Despite needing to resort to Miss Alexa’s and Miss Ceilidh’s bathroom for the morning, I’m glad ours at least smells of fresh, Pantene shampoo.

I continued my morning in the lab! Today’s procedure consisted of a transformation where we got to make more hopefully resistant Vibrio natriegens by making the bacteria take up the resistance gene. After hitting Dr. Duffy with the ✌ , I recently learned from him that throwing up the peace sign translates to the middle finger back in Scotland (oops). So to all the Scottish people I threw peace signs at in my life, I only mean love!!

After lab, I headed off back to Pinon Hall for my routine beauty nap. It was much needed rest for the unexpectedness of Miss Ceilidh’s and Miss Alexa’s TA talks.

Being born and raised in Texas, you would think I’d have the art of line dancing instilled in me since birth. Let me just say that is definitely not the case. Miss Alexa’s TA talk consisted of teaching us how to line dance where we learned line dancing to “Shivers” and “Cotton Eye Joe” With all its repetitive movements, I did eventually get the hang of it.

We were not done with dancing yet. Miss Ceilidh TA talk was learning a choreographed dance to the song “Yeah” by Usher, one of the best songs to jam out to I will say.

Our sweaty selves walked over to the lecture room for our guest speaker, Dr. Juliet Johnston where she talked about microbial wastewater treatment and queer science.

It was then dinner time. Over these five weeks, I eventually gravitated to these three foods: french fries, grilled chicken, and ice cream. A balanced meal I would say! I sat with Angelina, Eva, and Mr. Joey where we discussed fraternities and places we’ve been to.

We returned back to the lab after dinner to finish off our transformation experiment where Michael went crazy with the ethanol bottle and we drew a Rolex on Hugo’s lab coat.

Also spent time signing other people’s lab coats. A bittersweet moment indeed. Realizing that we officially only have 5 days left here, I am reminded of how much I won’t miss the dry heat or the cockroaches scattered across our dorms, but instead, the people I have gotten to know so well over these short five weeks.

The rest of the night was spent grinding out our posters and manuscripts. Every few minutes, it’s essential that I brain rot from the chaoticness of all this work and I somehow end up at the vending machine on the first floor of Foster. I have made so many mindless trips to the vending machine that I have discovered the perfect vending machine combo. Twix and cookies and cream Hershey bars. Thank you to the big backs, Kano and Arthur, for putting me on <3.

I walk back to the dorms with a sinking feeling. Not because I just consumed three Hershey bars, but because I have one day less to be with my closest friends.

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NMSU Genomics – Day 23: Angelina’s Blog https://summerscience.org/nmsu-genomics-day-23-angelinas-blog/ Wed, 31 Jul 2024 07:36:58 +0000 https://summerscience.org/?p=23285 ᴺᴼᵂ ᴾᴸᴬᵞᴵᴺᴳ : my way by frank sinatra ᴠᴏʟᴜᴍᴇ  : ▮▮▮▮▮▮▯▯▯ 0:57 ━━━•──────── 3:59 ⇄  ◃◃   ⅠⅠ   ▹▹  […]

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ᴺᴼᵂ ᴾᴸᴬᵞᴵᴺᴳ : my way by frank sinatra

ᴠᴏʟᴜᴍᴇ  : ▮▮▮▮▮▮▯▯▯

0:57 ━━━•──────── 3:59

⇄  ◃◃   ⅠⅠ   ▹▹  ↻

“It’s time to go to Carlsbad!” is what I wished I would’ve thought when I woke up this morning. Like an SNP being the difference between antibiotic resistant bacteria and non-resistant bacteria, one might even say, a singular event can be the difference between a broken heart and an intact heart. (Queue “Hundred Broken Hearts” by ENHYPEN) Even if we can’t go, the TAs and Mr. Steinman are plotting a bombastic and chocked full of activities Saturday for us, so I’ll trust the process. One of the things I’ve had to learn at SSP is the ability to adapt, just like our lovely Vibrio natriegens. Sacrificing breakfast in exchange for sleep has become a common practice that’s enabled by my roommate Emily, who also enjoys waking up later. Being the vampire (New Yorker) that I am, I always have an umbrella against the New Mexico rays that reach UV values over 10. And so, umbrella in hand, I walked to Foster Hall to start another day at SSP (Summer Sleeping Program). 

There’s never a dull moment in the superior lab group Fanum Taxonomy. I mean, what other group is burning a hole through their lab coat with a loop, sticking their finger into a bacterial plate, getting their hair singed off by a bunsen burner, and inhaling ethanol until it burns our eyes and lungs? Today, we spent the morning in the lab doing PCRs. Since we can’t go a day without being silly, we happened to forget which primers our group was given, but thanks to Ms. Ceilidh, we eventually figured it out. 

We all then headed down to the dungeon/lecture room to intensively work on our bioinformatics, research papers, and posters. As any good student knows, though, the brain needs breaks for efficiency’s sake. Thus, we embarked on our usual brain refreshing activities that range from practicing on monkeytype to playing Brawl Stars to watching beautiful, youthful men dance and sing. Between Vageesh’s manic mutation hunt with our glorious king Mr. Shakil’s tutelage and my side quests (much to Chloe’s dismay), our poster is looking a tad bit spacious, but fear not! Throughout heaven and earth, locked in Fanum Taxonomy alone is the honored one.

Instead of going to the dining hall, we picked up packaged lunches and headed back to the dorms. Efforts to organize dance practices for the impending talent show during lunch breaks have sadly been futile. Arion, Klara, Ryan, and I were planning to dance to “Magnetic” by ILLIT, but the Haidilao dance and “Good night, ojou-sama” by ASMRZ have been distracting us. Perhaps we’ll add it in? Writing a rap to “Not Like Us” by Kendrick Lamar might be the move (sorry Drake fans). Our talent show is on the upcoming Monday, and I can’t wait to see what my fellow participants have cooked up the past six weeks. I’ve heard rumors of a Tequila performance and a spreading session. Dr. Duffy was singing praises about the talent show last year, so I’m fully prepared to blow him away. If all goes wrong, we can just say it was a comedy act; we’ll be entertaining for sure.

Our work throughout the rest of the day consisted of rotating through steps for our transformation using known antibiotic resistance genes and continuing to procrastinate on our research paper and poster. Well, some groups were locked in. We just didn’t happen to be one of those groups.

Mr. Steinman, per his wife’s order, bought us Papa John’s pizza for dinner to reward us for our hard work. He unfortunately laced our dinner with a physics lesson, where he discussed Gauss’s Law and other topics my bio brain can’t comprehend. It was my first time trying Papa John’s, and since I hadn’t eaten anything throughout the day, I was definitely a big back. Fanum Taxonomy is still waiting on the ice cream that Dr. Catalan promised. A while ago, during the time our chemostat was exploding every day, Dr. Catalan said he would buy us ice cream if it exploded again. Thankfully, or maybe not thankfully, it did explode. He has also mentioned getting tacos for everyone, so it seems Dr. Catalan’s wallet may soon face an economic downfall. I hope the benevolent higher ups of SSP can cover this for him.

After another day of finishing up late, I walked back to the dorms with Ritisha. We ran a bit, which is uncommon for a League of Legends player like myself, to catch up with Penichillin plus Arion, but only Klara waited for us. The others say I have a slow walking pace, but I think I’m just enjoying my surroundings. The number of skunks I’ve seen in New Mexico is probably more than the sum of skunks I’ve seen in my life prior to SSP. The  resident cockroaches also come out at night, giving us the occasional fright on our walks. Though some may say there’s not a lot to do in Las Cruces, the lack of light pollution lets you see the sky clearly. I even saw a shooting star once here. Wondering what my wish was? It’s a secret. 

I dropped off my bookbag in my dorm before heading out on a walk with Ritisha, Ryan, and Vicky. Our TAs were feeling kind, so they extended our curfew from 10:00 pm to 11:00 pm. We made a trek to Garcia Hall just a few minutes away. The vending machines in Foster are depleted of our beloved Hershey’s Cookies ‘n’ Creme, but the Garcia machines were luckily stocked up. Though I usually swear off caffeine and rely on willpower, I felt like indulging in a Starbucks Mocha bottle. 

Vicky and Ritisha were feeling sleepy, so we walked back with them before we ran into Arion. Thus, Arion, Ryan, and I took another walk. According to my health app, my lowest steps per day the past year was in December, where I averaged approximately 2,200 steps a day. Yikes, I know. Here, I’m averaging about 10,000 steps per day. SSP (Summer Steps Program) is really getting us nerds into shape. 

Once the clock struck 11:00 pm, we dashed to our rooms for the room checks, wrapping up our second to last Wednesday on July 24th, 2024. 

Side note: I ended up not sleeping at all. My willpower combined with caffeine is too powerful.

Miss Ceilidh said I could do some side quests after I blog about my day, so here I go. 

I was going to make a tierlist of the participants, but I figured that’d be unethical. Instead, I’ve decided to do superlatives. Please keep in mind this was a joint effort and many opinions were included. 

We almost never know when something will be our last. White Sands became our last field trip after Carlsbad was canceled. Our last Target run was our last Target run and maybe the cockroach you killed today was the final cockroach of your stay here, or maybe even today was the last time we’ll ever hear Dr. Duffy scold us for being late to class. I surely hope today was the last impromptu physics lesson from Mr. Steinman. Jokes aside, every moment here feels precious, and I’ll genuinely miss everyone. Thus, I’ll have no shame in being sappy. I may never have the chance to be sappy again. 

To any parents, I love your child. I love you for creating and raising my friends. Sorry if that sounded weird. To any future applicants or participants, trust the process. To any of my fellow participants, I love you. Let’s meet again one day. To any faculty that may be reading this, I love you. Thank you for everything. To any other individual reading this, how are you still here? I appreciate it, though. 

Until we meet again,

Angelina 

✧・゚: *✧・゚:*

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NMSU Genomics – Day 22: Eva’s Blog/Vlog https://summerscience.org/nmsu-genomics-day-22-evas-blog-vlog/ Mon, 29 Jul 2024 04:16:39 +0000 https://summerscience.org/?p=23257 We all know the expression: “last night was lit, crazy movie”. Little did I know that when coming to SSP, […]

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We all know the expression: “last night was lit, crazy movie”. Little did I know that when coming to SSP, that every day would become a lit, crazy movie. 

Each and every day here at SSP there’s a rising action, a build up of suspense or pressure (will the chemostat tubing explode again?! Have our bacteria successfully gained spec resistance?! Will our nanodrop DNA be decently concentrated?!), and at last: a climax and resolution. 39 days in retrospect seems like a lot, but every one is valuable and every one is unique. 

Today, the 31st day of the program, my friends and I learned about how to model 3D protein structures for enzymatic subunits in Swiss Model. However, we also set out on a crusade in which we yet again conquered the Panda Express menu, putting our lives in the hands of fate as we cracked open our slightly too-pale cookies and read the red fortunes within. Though many of us were stuck in the lecture room working away at our posters until late in the night, the work itself blended into a backdrop of collaboration, laughs, and curiosity.

Enjoy the vlog!

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NMSU Genomics – Day 21: Ryan’s Blog https://summerscience.org/nmsu-genomics-day-21-ryans-blog/ Wed, 24 Jul 2024 16:10:33 +0000 https://summerscience.org/?p=23058 “Time flies when you’re having fun” – anonymous Today marks the first day of the last full week of SSP. […]

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“Time flies when you’re having fun” – anonymous

Today marks the first day of the last full week of SSP. The beginning of the end. During the first week of SSP, one of our TA’s, Miss Alexa, told us that the days would go by slowly, but the weeks would go by quickly. She was right – for the first few weeks. Now, it feels like the days and weeks are flying by, leaving me no time to stand by and enjoy the scenery. Today was no different.

“Maybe the real treasure was the friends we made along the way” – me (perchance)

My breakfast/lunch table is comprised of eight people, an entire third of the program eating and spending quality time with each other. Today, we had three people. Not because there’s beef going on, not because there was a complication or issue, but because five of our eight people were deep in hibernation like the sleepyheads they are. However, Ritisha, Klara, and I made the most of our breakfast, and Klara and I even managed to beat Ritisha in the NYT Mini with a time of 10 seconds!!! (Ritisha actually mogged us but we don’t talk about that)

How do antibiotics like to spend their time? Peni-chillin’!

Today was marked by a lack of lab time. Our schedule was filled to the brim with lectures, notifying us of an informational, yet unremarkable day ahead. However, our “lectures” turned out to be embedded with lots of time to work on our posters and papers, giving us some academic freedom in the lecture room. Before we got to working, however, Dr. Duffy announced that the company that sequenced our genomes had not sent us our raw genome reads, so we would have to use the polished, clean reads that they had already assembled for us instead. We were very sad and devastated at the news that we wouldn’t have to spend more time using Galaxy. Another announcement we found out about the genome reads was that out of the two control genomes that were sent for sequencing, one of them had been mislabeled and was actually from a vial with antibiotic. This meant that there was only one applicable control genome remaining, and it was a genome extracted from my group’s vial! This means that my group, Penichillin, will be on everyone else’s paper as an acknowledgement 😌. Common Penichillin W tbh.

If I had a nickel for every time I learned an official Kpop dance with a group of nerds, I’d have two nickels. Which isn’t a lot, but it’s weird that it happened twice.

Lunchtime was as usual – from 11:15 to 12:45. Today, we decided we needed to get on the grind. So after devouring our lunch, Klara, Angelina, Arion, and I headed back to the Piñon Hall common room for an intensive practice session. It was finally time to lock in and learn the dance for Magnetic, a Kpop song by the group Illit. We’d previously practiced the choreo for the first chorus of the song, but obviously that isn’t good enough for the talent show. While the dance is definitely difficult, I listened to the wise wise words of Justin Bieber and never said never. As I like to say, anything is possible if you just believe in yourself. 

“Let me show you how to blast” – Ritisha Dey

Our afternoon was spent doing two things: our problem set and BLASTing. NCBI’s BLAST feature may be really useful, but that doesn’t mean it didn’t make me want to drop out of school and become a Twitch streamer. We spent hours trying to figure out how to use BLAST, along with Galaxy, to find the mutations in our genomic sequences. Tears were shed, despair was rampant, and our hopes and dreams were being crushed. However, after many iterations of BLASTing, we had a semblance of a start to our genomic analysis process (special shoutout to Ritisha for being a master blaster and helping out). The light at the end of the tunnel was getting brighter, and when we found our first relevant mutation in DNA gyrase, we discovered how Newton felt when he invented gravity for the first time (isn’t it crazy how people survived without gravity before Newton invented it?). 

“It’s not about the destination, it’s about the journey” – Also me, perhaps

Today was exceedingly average. We didn’t have an exciting field trip, a birthday, a shopping run, or a movie. However, it’s these days that portray the true essence of SSP. The days we spend at the mall, White Sands, or the baseball game will stand out, but each day that passes where we laugh and joke while we work on Galaxy, each day where we blast music in the study room, and each day where we look up at the moon while walking and talking on the way to the dorm are invaluable. 

“Sometimes you will never know the value of a moment until it becomes a memory” —Theodor Seuss Geisel

Figure 1. Walking to breakfast!
Figure 2. Galaxy and blasting
Figure 3. The master blaster
Figure 4. Walking back in the night

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NMSU Genomics – Day 20: Jude’s Blog https://summerscience.org/nmsu-genomics-day-20-judes-blog/ Mon, 22 Jul 2024 20:30:11 +0000 https://summerscience.org/?p=22928 I woke up a bit later than usual, around 8:10, because it was Saturday. A lot of us were wearing […]

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I woke up a bit later than usual, around 8:10, because it was Saturday. A lot of us were wearing shorts and/or sandals since we were done with all labs for that week! Anyways, I walked to get breakfast with Ritisha in Foster Hall. Like always, it was bagels and donuts with coffee. Afterwards, we walked downstairs to the lecture hall for a really interesting lecture over cultural capital. Dr. Catalán claimed that the slideshow came from the University of Michigan, but any self-respecting Hoosier would have recognized the crimson and cream logo of IU on it. Afterwards, we got back to the dorms around 10 and got to rest for a bit until lunch. I practiced my piano piece, Chopin Etude Op. 25 Nr. 1, for the talent show.

I had previously thought the NMSU bookstore was closed because it was the weekend, but I think it was open due to freshman orientation. So, I asked Ritisha to come with me to get my daily Starbs. I got a venti Mango Dragonfruit Refresher rather than my usual crème-based Vanilla Bean Frappuccino order. Then, my friends and I planned out our mall trip, a pleasant surprise from our wonderful TAs! But before we went, we got lunch—I had their pasta.

After we returned to the dorms to prepare for the mall trip, we made our way over to the bus stop next to Piñon Hall and had a rather interesting conversation with Vageesh about my Indian friends back at home… Anyways, we boarded the bus and set off for the Mesilla Valley Mall. We had two hours and two hours only there—I intended to make the most of them. My friends and I first stopped by at Auntie Anne’s, where I got a large cup of cinnamon pretzel bites and a lemonade, both of which I promptly scarfed down. I did a challenge with some friends in H&M in which we were each assigned to dress up another person. Maahi’s outfit absolutely ruined me, but at least I got to do the same to Ryan! After that, I got some really mid boba milk tea. We sauntered around the mall for a bit before I saw some crocheted plushies. I got a blue-purple octopus. Afterwards, we all met up at the end of our mall trip and showed each other our hauls. We got back to the dorms a little before dinner.

Dinner was better than usual because the incoming freshmen were there. I got potato chips topped with Tajín and lime juice. I sat with Mr. Shakil, and we had an interesting talk about Bangladesh, where he’s from, as my dad grew up near there in Kolkata. Afterwards, we got to return to dorms instead of having to do p-sets!

Sarah, Eva, Chloe, and Delilah wanted to watch a K-Drama, but we weren’t able to access Netflix on the TV in the common room. The remote control has been missing for a while, but I was able to connect using my phone with the help of an Astro participant. I was only able to login to my Hulu account, so instead of Crash Landing on You or Business Proposal, we had to watch What’s Wrong with Secretary Kim? We got comfy with blankets, soda, gummy bears, potato chips, and more food. One episode in, however, Eva got up to get more food, and when she came to sit back down, she hit the remote, and the TV turned off. We weren’t able to turn it back on…

So, we were in the room with the lights turned off and no K-Drama to watch—we decided to take a nap instead! We put on soothing sleeping  music and tried to sleep. Whenever someone entered our room, though, we couldn’t stop laughing. Later on in the night, Ms. Alexa taught some other people line dancing, and a mini-concert was held in the lobby. Then finally, it turned 11pm, so off we went to our rooms.

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NMSU Genomics – Day 19: Brett’s Blog https://summerscience.org/nmsu-genomics-day-19-bretts-blog/ Sat, 20 Jul 2024 11:00:00 +0000 https://summerscience.org/?p=22840 Half Birthdays and Whole Genome Sequencing Analysis Although I wholeheartedly believe that genomics is superior to astrophysics, today was the […]

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Half Birthdays and Whole Genome Sequencing Analysis

Although I wholeheartedly believe that genomics is superior to astrophysics, today was the first day at SSP when I woke up thinking about the stars. However, it wasn’t just any random star that caught my attention; I woke up thinking about the sun.

Today is my half birthday, which means I have officially traveled around the sun 17.5 times. That’s ½ a revolution (or 648,000 arcseconds in astro-speak) away from 18. Not only is 18 my favorite number, but also it’s the day of the month that I was born. Although I don’t think about astronomy often, the stars were definitely on my side on July 18th at SSP.

Last night, we had the incredible opportunity to travel to White Sands National Park at sunset. In addition to taking a gargantuan amount of pictures (thanks Emily), we sat and talked as the sun went down. Hugo and I attempted sledding down the sand dunes, yet we were largely unsuccessful. 

Caption: Us featuring Dr. Catalan’s son Dante 😊

After sleeping through breakfast this morning (per usual), my roommate Vageesh, Kano, and I walked to Foster Hall. As we arrived two minutes before lab, we were greeted with an unpleasant surprise: the AC in the lab was broken. Fortunately, our DNA samples had been sent off for sequencing, so we were able to enjoy the pleasant cool air of the lecture room for the majority of the day. Nevertheless, we still had to take apart our chemostats and set them up for autoclaving. 

Following three arduous weeks of blood, sweat, and tears working with and repairing our chemostats, it wasn’t very tough to say goodbye. I think I speak for my fellow members of lab group W. rizz when I say that chemostat SSP14 caused more pain than joy. With tough times comes great reward, and I am incredibly excited to get our genome sequencing results back soon.

After a great lecture by Dr. Messner on writing the “Materials and Methods” section of our manuscripts, I walked to Taos for lunch. Today they served coconut rice, which reminded me of one of my favorite restaurants from home. I have yet to try authentic New Mexican food here in Las Cruces, and I remain hopeful that Dr. Catalan follows through on his promise of giving us a taste.

Because our wet lab experience has mostly come to a close, our post-lunch game plan consisted of everything bioinformatics. My lab mate Delilah and I tried to keep our eyes open as we parsed through endless tabs of the NCBI database, Minimap2, Flye, and Google searches on how to convert a fasta file to PFA. I felt like I was bordering on Minia (Galaxy users will get the pun). Due to our persistence and determination, we were able to get it done in the end.

Caption: Galaxy ft. Chloe, Vageesh, and Eva

At dinner, I had the privilege of having another great talk with Dr. Messner, Kano, and Hugo. From funny stories about childhood to deep talks about family and religion, I always leave the table with a smile on my face. Though it was our last meal together, I am excited to see what the next seating chart has in store. 

Caption: Dr. Messner, Kano, Hugo, and me at dinner

Many moments later, I traversed back to Foster to finish my problem set and write this blog post. The sun is about to set over the adobe clay roofs of New Mexico State University, and the wind is starting to sway the palm trees side to side. Sunset usually means the bugs start to come out, but I’m luckily equipped with plenty of bug spray. Despite the assortment of bug bites lining my ankles, the glass is always half full at SSP.

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NMSU Genomics – Day 18: Sarah’s Blog https://summerscience.org/nmsu-genomics-day-18-sarahs-blog/ Fri, 19 Jul 2024 18:00:00 +0000 https://summerscience.org/?p=22826 hey!!  Our genomics group goes on field trips every Wednesday so I decided to film clips from throughout the day […]

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hey!! 

Our genomics group goes on field trips every Wednesday so I decided to film clips from throughout the day and make a vlog! 

I got to sleep in after a long night in the lab (it was our last time working with the chemostats). My friends and I went to panda express and starbucks for brunch, and then explored our campus bookstore. In the afternoon, the TAs (miss Alexa and miss Ceilidh) and Dr. Messner took all the girls to the craft store to make friendship bracelets. The highlight of our day though was definitely going on a trip to white sands! The view was amazing and everyone took really cool photos.

i hope you enjoy the vlog!!!

Sarah <3

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NMSU Genomics – Day 17: Delilah’s Blog https://summerscience.org/nmsu-genomics-day-17-delilahs-blog/ Fri, 19 Jul 2024 12:00:00 +0000 https://summerscience.org/?p=22793 This morning I woke up early to shower at 6:30! Me and my friends headed over to the dining hall […]

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This morning I woke up early to shower at 6:30! Me and my friends headed over to the dining hall and we had potatoes, sausage, chocolate chip pancakes, and eggs with cheese for breakfast.All lab groups started our day off in the lab where we checked our plating from the previous night. We plated samples from our experiment vials to see if our bacteria had gained any antibiotic resistance. We spent some time in the lab and we started to work on a few of our other things. We then had to repurify our DNA because the concentration was not pure enough from the first step of purification, which was kind of annoying, but it’s part of biology! Since we had to repurify our DNA, my group W. Rizz went to lunch a little late but we still had plenty of time.  My best friend called me and I talked to her for a while. I had my favorite drink from the dining hall, cranberry juice and a little bit of water. I got a caramel macchiato from the bagel place outside of the dining hall and it was yum! It was around 12: 45 and we had to go to our lecture for the day. For the lecture today we had to analyze the methods and materials section for various research papers. We analyzed, and it was a total of eight papers that we looked at between the twenty-four of us. And then we analyzed them and talked about the differences and the similarities. And then we went back to our group and started working on our own materials and methods. After finishing that and then we had to perform a presentation on our antibiotic resistance. For the presentation we had to compare our previous plates from the first week to our plates after we put them into the chemostat for about a week and a half. My lab group has been working with tetracycline but we also looked at other ones for ciprofloxacin and erythromycin. And then we went back to the lab and we had to take pictures of our plates again oh wait go back. For the presentation the professors choose someone random from each group and I happen to be the random person and I was super nervous to present, but then my friends started clapping for me as I was walking up before I even presented and then I smiled, and everyone started clapping and made me a little bit less nervous. We’re in the lecture room until around 3 o’clock for the presentations and then we went back upstairs to check on the lab briefly. Later in the afternoon we had a guest speaker, Dr. David Baltrus and he talked about antibiotic resistance and genomics within the realm of agriculture. The group that introduced him lit up the room with glow sticks, and it was a great way to start the lecture. After we had some cookies and drinks, we all sat around Dr. Baltrus asked him a few questions and he answered them very happily. He told us about his work life, as well as some of his home life as well. He spoke to us about his journey to his PhD and his lab. Unfortunately he was unable to have dinner with us as he told us he was gonna be on a 30 hour car ride to somewhere in South Carolina.  Dinner that day was fried fish tacos and I don’t like fish so I had chicken nuggets and fries instead. But they don’t call it chicken nuggets, they call it tempura fried chicken!  I also got some ice cream with my friends. We have ice cream like every day and I never really ate ice cream before this but now it’s basically a daily staple. I sat with Mr. Steinman, our site Director at dinner today and we talked about so many things.  Some conversation topics included whether if hotdogs are sandwiches, does that mean that a taco is a sandwich as well?We got to talking about superpowers and he was ecstatic to tell us all the bad sides of each superpower. He also told us that once he did it for two hours in his college dorm room. We also talked about how I don’t know any geography, which started as him being shocked I don’t know where Pennsylvania is. He asked me to do my ride from my left and I put an “L” up with my left hand and he started laughing like it was the funniest thing ever. After dinner, my lab group was done with our work so I went back to the dorm and I just kind of chilled out for a little bit. I spent some time calling my mom and I was on TikTok for a little bit and then I went out to the common room where I saw a couple of friends we walked around and looked at the sunset, which is very nice. The New Mexico sunsets are unmatched. I spent the rest of the night just hanging out with my friends and talking to them. A lot of people are preparing for the talent show already practicing what they’re gonna do, whether it be piano, singing, or speech and debate spreading. The night came to a close and I walked back to my dorm with my friend.

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NMSU Genomics – Day 16: Chloe’s Blog https://summerscience.org/nmsu-genomics-day-16-chloes-blog/ Thu, 18 Jul 2024 22:33:21 +0000 https://summerscience.org/?p=22791 WE GOT COLONIES! It was 6:10 AM on July 16th. After reautoclaving our chemostat seven times, switching our antibiotic (our […]

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WE GOT COLONIES!

It was 6:10 AM on July 16th. After reautoclaving our chemostat seven times, switching our antibiotic (our original antibiotic gentamicin was faulty), and incubating our plates for 12+ hours, we finally got colonies! Now, we have to prepare our samples for sequencing.

This all started the day before: July 15th.

This morning, I finally mustered up all my strength to get out of bed at 7:20, 35 minutes after my first alarm… it doesn’t matter how early I go to sleep, I never wake up when my alarm goes off. I headed over to breakfast with a few of my friends. 

Breakfast was average: nothing too special, but it was still satisfying. At about 8:10, it was time to head to Foster Hall, where lab and lecture are held.

It is always super hot and dry, but since we are in the lab everyday, we unfortunately have to wear long pants. 

I walked into lab this morning to see that my waste bottle was overflowing. Waste from our vials in our chemostat was quite literally dripping out onto the table. The stench of the waste is indescribable. We were luckily able to pour it out and all was good. We were praying that this round would be successful and we would have resistant bacteria. 

We all headed to the lecture room in the basement and worked on our introductions for a while. We made some progress. Not really too much though. We were occupied with monitoring our chemostat on replifactory, the website we had been using to monitor all the data our chemostat was measuring. Soon, it was time to go back to the lab to check on our chemostats. Many other groups began with testing their bacteria to see if they had become resistant. They did this by streaking them on an antibiotic plate to see if the bacteria would still grow. However, since we had just started our experiment less than 3 days ago, we couldn’t do that yet. Our bacteria weren’t ready. So instead of doing that, we were working hard on our introduction… and working on some typing. Recently, our entire program has begun to see how fast people can type (shoutout monkeytype). 

Finally, it was time for lunch, so we all headed back to the cafeteria. After a nice lunch (that I forgot to take a picture of), I walked out to see a skunk right outside the window. 

Skunks are everywhere here. Going back to the dorms at the end of the night, it is almost inevitable to see at least one.

Soon, we were all back in the lab for the rest of the afternoon. We began with gDNA extraction (using another lab group’s culture). 

While we were adding various buffers to the DNA and centrifuging, we were delivered the amazing news that we didn’t have any problem sets (psets) for the night and everyone jumped and screamed with joy. 

After a few hours of extracting DNA, my lab group (Angelina, Vageesh, and I) began to work on plating our cultures from our vials. The process was pretty long, but it was actually a lot of fun (other than the part where I stabbed myself)! Don’t worry, I did not inoculate myself with bacteria. The wound is already closed up.

We were the last lab group to leave the lab for dinner, but at least we finished streaking all of our plates! Now, all we had to do was pray that colonies would grow — this would mean that our bacteria was resistant. 

My dinner table: me, Delilah, Vicky, and Mr. Steinman (left to right)

Right after dinner, we headed right back to lab. As you can tell, we spend A LOT of time in the lab. Angelina, Vageesh, and I had to replace our media bottle and drug bottle for our chemostat. Honestly, this was unnecessary since we had already taken our samples, but we couldn’t just leave our vials with no media or drug.

By this time, we were beginning to realize that we wouldn’t be able to send our sequences tomorrow if we started the day at our regular hours, so we made a plan! Assuming our bacteria was resistant, we, along with the Streakers (Sierra, Arthur, and Hiram), convinced our advisor/TA Ms. Ceilidh to let us come to the lab at 6 AM the next morning (7/16) to pick colonies off our plates and as you know, THEY GREW.

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